Osong Public Health and Research Perspectives

Original Articles

Genetic Variability of Methicillin Resistant Staphylococcus Aureus Strains Isolated from Burns Patients: Mehdi Goudarzi, Nobumichi Kobayashi, Ali Hashemi, Maryam Fazeli, Masoumeh Navidinia; Osong Public Health Res Perspect. 2019;10(3):170-176. Published online June 30, 2019; DOI: https://doi.org/10.24171/j.phrp.2019.10.3.08

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Objectives

Staphylococcus aureus is a nosocomial pathogen that provides a major challenge in the healthcare environment, especially in burns units where patients are particularly susceptible to infections. In this study, we sought to determine molecular types of S. aureus isolates collected from burns patients, based on staphylococcal protein A and coagulase gene polymorphisms.

Methods

Antibiotic susceptibility testing of 89 S. aureus strains isolated from burn wounds of patients was assessed using the Kirby-Bauer disk diffusion method. Strains were characterized by spa typing, coa typing, and resistance and toxin gene profiling.

Results

A total of 12 different spa types were identified with the majority being t790 (18%). Panton-Valentine leucocidin encoding genes were identified in spa types t044 (5.6%), t852 (2.2%) and t008 (2.2%). The most commonly detected antibiotic resistance gene was ant (4′)-Ia (60.7%). Ten different coa types were detected and the majority of the tested isolates belonged to coa III (47.2%). All the high-level mupirocin-resistant and low-level mupirocin resistant strains belonged to coa type III.

Conclusion

The present study illustrated that despite the high frequency of coa III and spa t790 types, the genetic background of S. aureus strains in Iranian burns patients was diverse. The findings obtained are valuable in creating awareness of S. aureus infections within burns units.

Citations

Citations to this article as recorded by

Vancomycin heteroresistance among methicillin-resistant clinical isolates S. haemolyticus, S. hominis, S. simulans, and S. warneri
Magdalena Szemraj, Paweł Lisiecki, Paulina Glajzner, Eligia M. Szewczyk
Brazilian Journal of Microbiology.2023; 54(1): 159. CrossRef
Emerging Challenges in Staphylococcus aureus Bloodstream Infections: Insights from Coagulase Typing, Toxin Genes, and Antibiotic Resistance Patterns
Samira Zamani, Masoud Dadashi, Sara Bahonar, Mehrdad Haghighi, Sareh Kakavandi, Ali Hashemi, Mohammad Javad Nasiri, Bahareh Hajikhani, Mehdi Goudarzi, Veronica Folliero
Advances in Medicine.2023; 2023: 1. CrossRef
Characteristic, antibiotic resistance and molecular typing of Staphylococcus aureus isolated from intensive care unit and burn patients based on coagulase gene analysis
Bahareh Hajikhani, Anis Mohammadi, Mohammad Javad Nasiri, Masoud Dadashi, Ali Hashemi, Mehrdad Haghighi, Mirmohammad Miri, Mehdi Goudarzi
Gene Reports.2022; 26: 101542. CrossRef
Efficacy of Phage- and Bacteriocin-Based Therapies in Combatting Nosocomial MRSA Infections
Lauren Walsh, Crystal N. Johnson, Colin Hill, R. Paul Ross
Frontiers in Molecular Biosciences.2021;[Epub] CrossRef
Hospital clones of Panton-Valentine leukocidin-positive and methicillin-resistant Staphylococcus aureus circulating in the Tehran community
Samira Tajik, Shahin Najar-Peerayeh, Bita Bakhshi
Journal of Global Antimicrobial Resistance.2020; 22: 177. CrossRef
Characterizing a Lytic Bacteriophage Infecting Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Burn Patients
Masoume Hallajzadeh, Ali Mojtahedi, Nour Amirmozafari, Vahid Pirhajati Mahabadi
Archives of Clinical Infectious Diseases.2020;[Epub] CrossRef
Methicillin-Resistant Staphylococcus aureus ST80 Clone: A Systematic Review
Assia Mairi, Abdelaziz Touati, Jean-Philippe Lavigne
Toxins.2020; 12(2): 119. CrossRef
The prevalence and molecular mechanisms of mupirocin resistance in Staphylococcus aureus isolates from a Hospital in Cape Town, South Africa
Shima M. Abdulgader, Tshepiso Lentswe, Andrew Whitelaw, Mae Newton-Foot
Antimicrobial Resistance & Infection Control.2020;[Epub] CrossRef
Search of Potential Vaccine Candidates against Trueperella pyogenes Infections through Proteomic and Bioinformatic Analysis
Ángela Galán-Relaño, Lidia Gómez-Gascón, Antonio Rodríguez-Franco, Inmaculada Luque, Belén Huerta, Carmen Tarradas, Manuel J. Rodríguez-Ortega
Vaccines.2020; 8(2): 314. CrossRef
Genotypic and Phenotypic Characterisation of Clinical Isolates of Methicillin-Resistant Staphylococcus aureus in Two Different Geographical Locations of Iran
Shiva Ahmadishoar, Nadia Kazemi Pour, Javid Sadeghi, Mohammad Reza Nahaei, Babak Kheirkhah
Indian Journal of Medical Microbiology.2020; 38(2): 162. CrossRef

Natural Infection with Rabies Virus: A Histopathological and Immunohistochemical Study of Human Brains: Firouzeh Farahtaj, Leila Alizadeh, Alireza Gholami, Alireza Tahamtan, Sadegh Shirian, Maryam Fazeli, Amir Sasan Mozaffari Nejad, Ali Gorji, Hamid Mahmoudzadeh Niknam, Amir Ghaemi; Osong Public Health Res Perspect. 2019;10(1):6-11. Published online February 28, 2019; DOI: https://doi.org/10.24171/j.phrp.2019.10.1.03

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245 Download
12 Crossref

Abstract PDF

Objectives

Despite all the efforts and increased knowledge of rabies, the exact mechanisms of infection and mortality from the rabies virus are not well understood. To understand the mechanisms underlying the pathogenicity of rabies virus infection, it is crucial to study the tissue that the rabies virus naturally infects in humans.

Methods

Cerebellum brain tissue from 9 human post mortem cases from Iran, who had been infected with rabies virus, were examined histopathologically and immunohistochemically to evaluate the innate immune responses against the rabies virus.

Results

Histopathological examination revealed inflammation of the infected cerebellum and immunohistochemical analyses showed an increased immunoreactivity of heat shock protein 70, interleukin-6, interleukin-1, tumor necrosis factor-alpha, caspase-3, caspase-9, toll-like receptor3 and toll-like receptor4 in the infected brain tissue.

Conclusion

These results indicated the involvement of innate immunity in rabies infected human brain tissue, which may aggravate the progression of this deadly disease.

Citations

Citations to this article as recorded by

Molecular Mechanisms Associated with Neurodegeneration of Neurotropic Viral Infection
Prapimpun Wongchitrat, Theerawut Chanmee, Piyarat Govitrapong
Molecular Neurobiology.2024; 61(5): 2881. CrossRef
Systematic development of immunohistochemistry protocol for large cryosections-specific to non-perfused fetal brain
Karthika Pandurangan, Jaikishan Jayakumar, Stephen Savoia, Reetuparna Nanda, S. Lata, E. Harish Kumar, Suresh S., Sudha Vasudevan, Chitra Srinivasan, Jayaraj Joseph, Mohanasankar Sivaprakasam, Richa Verma
Journal of Neuroscience Methods.2024; 405: 110085. CrossRef
Biosensor as an alternative diagnostic method for rabies virus detection: A literature review
Milad Zandi, Sajad Zandi, Ramin Mohammadi, Parastoo Hosseini, Samane Teymouri, Saber Soltani, Azadeh Rasouli
Biotechnology and Applied Biochemistry.2022; 69(4): 1348. CrossRef
Immunohistochemical diagnosis of human infectious diseases: a review
Hamadou Oumarou Hama, Gérard Aboudharam, Rémi Barbieri, Hubert Lepidi, Michel Drancourt
Diagnostic Pathology.2022;[Epub] CrossRef
Mitochondrial Dysfunction in Rabies Virus-Infected Human and Canine Brains
Pulleri Kandi Harsha, Sathyanarayanan Ranganayaki, Gowri Yale, Gourav Dey, Kiran K. Mangalaparthi, Anusha Yarlagadda, B. K. Chandrasekhar Sagar, Anita Mahadevan, M. M. Srinivas Bharath, Reeta S. Mani
Neurochemical Research.2022; 47(6): 1610. CrossRef
A rare fatal case of rabies coexisting with COVID-19
RabiNarayan Hota, Shalendra Singh, Rakesh Sharma, Pallavi Khandare
Journal of Acute Disease.2022; 11(3): 129. CrossRef
Enhancement of immune responses by co-stimulation of TLR3 - TLR7 agonists as a potential therapeutics against rabies in mouse model
Firouzeh Farahtaj, Alireza Gholami, Mohammad Sadeq Khosravy, Safoora Gharibzadeh, Hamid Mahmoudzadeh Niknam, Amir Ghaemi
Microbial Pathogenesis.2021; 157: 104971. CrossRef
Establishment of Human-Induced Pluripotent Stem Cell-Derived Neurons—A Promising In Vitro Model for a Molecular Study of Rabies Virus and Host Interaction
Thanathom Chailangkarn, Nathiphat Tanwattana, Thanakorn Jaemthaworn, Sira Sriswasdi, Nanchaya Wanasen, Sithichoke Tangphatsornruang, Kantinan Leetanasaksakul, Yuparat Jantraphakorn, Wanapinun Nawae, Penpicha Chankeeree, Porntippa Lekcharoensuk, Boonlert L
International Journal of Molecular Sciences.2021; 22(21): 11986. CrossRef
Beneficial and Detrimental Effects of Regulatory T Cells in Neurotropic Virus Infections
Malgorzata Ciurkiewicz, Vanessa Herder, Andreas Beineke
International Journal of Molecular Sciences.2020; 21(5): 1705. CrossRef
Characterization of the Th17 profile immune response in cases of human rabies transmitted by dogs and its interference in the disease pathogenesis.
L.B. Santos, F. Guedes, S.M. Achkar, M.I.S. Duarte, I.S.S. Katz, S.R. Silva, E.R. Fernandes
Journal of Neuroimmunology.2020; 344: 577263. CrossRef
Quantitative proteomics leads to identify dog brain proteins involved in rabies virus infection: implication in understanding viral pathophysiology
Suchismita Behera, Rajesh Raghunath Pharande, R. Rajendra Reddy, Sharmila B. Majee, Sandeepan Mukherjee, Amol Ratnakar Suryawanshi
Journal of Proteins and Proteomics.2020; 11(4): 241. CrossRef
Feral dog bite causing paralytic rabies: Difficult diagnosis and failure of prevention
Hussein Algahtani, Bader Shirah, Emna Chtourou, Osama Abuhawi, Nawal Abdelghaffar, Mohammad Alshehri
Saudi Journal for Health Sciences.2020; 9(3): 260. CrossRef

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